Lifeboat Foundation

The human body is an incredible machine. It is impossible to determine which is the essential body part for sustaining life — because there is no single indispensable part. If your heart stops beating, you will die. If your lungs stop working, your brain — and thus all of your cells — will eventually die. Without a stomach or intestines you cannot acquire nutrients and you will die. All parts are critical for optimal function, for sustaining life.

Synthetic biology as a field is no different. There are those that supply DNA — arguably the critical building block for every single synthetic biology application. There are those that automate and scale components of the design-build-test cycle to enable innovation to effect change in meaningful timelines. But when all of those parts come together with a single goal, the power of synthetic biology reaches new levels.

Such potential is exactly what Arzeda — through a collaboration with TeselaGen, Twist Bioscience, and Labcyte — has brought to us. Each company, a giant in its own right, provides an essential, needed component to an elegant, efficient workflow that can best be described as a “DNA assembly line” for more rapid, efficient protein design and production. The companies’ products work seamlessly: Twist produces the DNA fragments needed to make protein-expressing plasmids, Labcyte’s acoustic liquid handler (the Echo 525) facilitates rapid DNA assembly, and TeselaGen’s DNA assembly design and laboratory automation software connects the two, designing plasmids and ordering the necessary sequences from Twist while generating worklists for the Echo to execute.

Truthfully, it has been some time since Moore’s law, the propensity for processors to double in transistor count every two years, has been entirely accurate. The fundamental properties of silicon are beginning to limit development and will significantly curtail future performance gains, yet with 50 years and billions invested, it seems preposterous that any ‘beyond-silicon’ technology could power the computers of tomorrow. And yet, Nano might do just that, by harnessing its ability to be designed and built like a regular silicon wafer, while using carbon to net theoretical triple performance at one-third the power.

Nano began life much like all processors, a 150mm wafer with a pattern carved out of it by a regular chip fab. Dipped into a solution of carbon nanotubes bound together like microscopic spaghetti, it re-emerged with its semi-conductive carbon nanotubes stuck in the pattern of transistors and logic gates already etched on it. It then undergoes a process called ‘RINSE,’ removal of incubated nanotubes through selective exfoliation, by being coated with a polymer then dipped in a solvent. This has the effect of reducing the CNT layer to being just one tube, removing the large clumps of CNTs that stick together over 250 times more effectively than previous methods.

One of the challenges facing CNT processors has been difficulty in separating N-type and P-type transistors, which are “on” for 1 bit and “off” for 0 bit and the reverse, respectively. The difference is important for binary computing, and to perfect it, the researchers introduced ‘MIXED,’ metal interface engineering crossed with electrostatic doping. Occurring after RINSE, small platinum or titanium components are added to each transistor, then the wafer is coated in an oxide which acts as a sealant, improving performance. After that, Nano was just about done.

We asked three centenarians what their most valuable life lessons were, and also their regrets.

The conversations that followed were remarkable. They talked about the importance of family, people, relationships and love. Their view on life, as an elderly citizen with a lot of experience is truly an inspiration and motivation. Enjoy the video!

Continue reading “Life Lessons From 100-Year-Olds” »

Most of my professional life is centered on synthetic biology, an industry and movement to make biology easier to engineer. So far, this emerging discipline has yielded everything from living medicines and spider silk jackets to impossible hamburgers. But what will humankind be growing in the next century?